1.0.0 CAM Assay Application Documentation
Application Name | CAM Grid Assay |
Version | 1.0.0 |
Documentation Version | 10.10.2019 - 1 |
Input Image(s) | 2D (standard) |
Input Parameter(s) | None |
Keywords | CAM, grid, onplant, chorioallantoic membrane, in-vivo, angiogenesis, chick, vessel, growth, microscopy, assay |
Short Description | Segmentation of new blood vessels on polymerized grids (“onplants”) placed on chick chorioallantoic membrane assay used in in-vivo angiogenesis research. |
References / Literature | For more information regarding the assay check e.g. https://www.ncbi.nlm.nih.gov/pubmed/19007659 |
Table of contents
IKOSA Prisma CAM Assay
You can use this image analysis application or any of our other applications in your account on the IKOSA platform. If it is not on the list of available applications, please contact your organization's administrator or our team at support@kmlvision.com.
Application description
This application automatically measures the number and sizes of blood vessels in microscopy images that are taken from CAM Assay samples. The application is optimized for detecting new blood vessels that grow through polymerized grids (onplants) placed on the CAM small vessels. Since the focus is on neoangiogenesis, larger vessels in the background are not detected.
In the following sections, we provide the necessary input data requirements that are necessary to obtain accurate image analysis results and a description of the output files.
Input data requirements
Input image(s)
Input for this application is the following image data:
Image type | Color channels | Color depth (per channel) | Size (px) | Resolution (μm/px) |
|---|---|---|---|---|
2D (standard)
Check image format https://kmlvision.atlassian.net/wiki/spaces/KB/pages/3406725124 | 3 (RGB) | 8 Bit | Min: 128 x 128 Max: 6680 x 5239 | Min: 0.8 Max: 1.2 |
Image content Microscopy image of CAM Assay region with typically 40x magnification and usage of polarization filter. Additional requirements
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Important:
For all images, the following requirements apply:
The illumination must be constant throughout the image(s).
The sample must be in focus, i.e. no blurry regions in image(s).
Input parameter(s)
No additional input parameters are required for this application.
Description of output files and their content
Files
File format | Description | |
|---|---|---|
| 1 | csv | results.csv A csv file containing the analysis results for the input image. |
| 2 | jpg | results_visualization.jpg A visualization of the detected vessels:
 |
Content
results.csv
Single csv-file
Column NO. | Column name | Examples | Value range | Description |
|---|---|---|---|---|
1 | Vessels_total_area [Px^2] | 3668 | 0 - #of pixels in image | Total area covered by detected vessels in Pixel^2. |
2 | Vessels_total_length [Px] | 12092 | >=0 | Total length of all detected vessels in pixels. |
3 | Vessels_mean_thickness [Px] | 6.98 | >=0 | Mean thickness of detected vessels in pixels. |
4 | vessels_num_branching_points | 776 | >=0 | Number of detected branching points.. |
Error information
More information about errors can be found in the Application Error Documentation.
Contact
If you have any questions about this app, as well as suggestions or ideas for new ones, email us at support@kmlvision.com.
Feel free to book a 30-minute meeting to speak with us about IKOSA and the apps!
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