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File formats

File formats

This table represents all image file formats that can be uploaded and viewed in IKOSA Portal.

Important:

  1. the maximum upload size of a single image is 16GB.

  2. not all file formats are supported by IKOSA Prisma and IKOSA AI image analysis apps. Please see the last column of the table below for more precise information.

  3. some applications may have additional image file requirements, i.e. not only format, but also bit/colour depth and size. These specifications are listed in the documentation of each application (IKOSA Prisma docs, IKOSA AI docs).

  4. some image types cannot be uploaded to IKOSA Portal, but they can be analyzed via Prisma API. All you have to do is contact us at support@ikosa.ai

Legend

purple - Whole-slide image support (also stated WSI)

Blue - standard image, no WSI support, maximum size of 625 Megapixel (e.g. 25,000 x 25,000 Pixel).

Type

File Extension(s)

Mime-Type

Supported by IKOSA Portal

Supported use (applications and training)

(IKOSA Prisma docs, IKOSA AI docs)

Type

File Extension(s)

Mime-Type

Supported by IKOSA Portal

Supported use (applications and training)

(IKOSA Prisma docs, IKOSA AI docs)

Aperio/Leica SVS (TIFF)

svs, tif

image/x.aperio.svs+tiff

2D - WSI - 8-bit

Applications:

Axon Quantification (>=1.0.0)

CAM Assay (>=2.0.0)

Confluence Assay (>=1.0.0)

Cytosolic & Nuclear Translocation Assay (1.0.0, 2.0.0, 3.0.0, 3.1.0)

Ki-67 Breast Quantification (>=1.0.0)

Network Formation Assay (>=2.0.0)

Neuronal Network Morphology (>=1.0.0) (not a publicly available app)

Spheroid Sprouting Assay (2.0.0, 2.1.0)

Wound Healing (Scratch) Assay (>=1.0.0)

Ovarian Carcinoma DAB Scoring (>=1.0.0) (not a publicly available app)

CD31 IHC Vessel Quantification (>=1.0.0)

Neurite Outgrowth PC (>=1.0.0)

IKOSA AI (deployed apps):

IKOSA AI Semantic Segmentation (>=4.2.0)

IKOSA AI Instance Segmentation 2D (>=1.0.0)

IKOSA AI (application training):

IKOSA AI Semantic Segmentation

IKOSA AI Instance Segmentation 2D

Hamamatsu NDPI

ndpi

image/x.hamamatsu.ndpi+tiff

2D - WSI - 8-bit

Applications:

Axon Quantification (>=1.0.0)

CAM Assay (>=2.0.0)

Confluence Assay (>=1.0.0)

Cytosolic & Nuclear Translocation Assay (1.0.0, 2.0.0, 3.0.0, 3.1.0)

Ki-67 Breast Quantification (>=1.0.0)

Network Formation Assay (>=2.0.0)

Neuronal Network Morphology (>=1.0.0) (not a publicly available app)

Spheroid Sprouting Assay (2.0.0, 2.1.0)

Wound Healing (Scratch) Assay (>=1.0.0)

Ovarian Carcinoma DAB Scoring (>=1.0.0) (not a publicly available app)

CD31 IHC Vessel Quantification (>=1.0.0)

Neurite Outgrowth PC (>=1.0.0)

IKOSA AI (deployed apps):

IKOSA AI Semantic Segmentation (>=4.2.0)

IKOSA AI Instance Segmentation 2D (>=1.0.0)

IKOSA AI (application training):

IKOSA AI Semantic Segmentation

IKOSA AI Instance Segmentation 2D

Zeiss CZI

czi

image/x.zeiss.czi

2D - WSI - 8-bit

2D - WSI - 16-bit

Multichannel - WSI - 8-bit

Multichannel - WSI - 16-bit

TIME SERIES - WSI - 8-bit

TIME SERIES - WSI - 16-bit

Applications:

Axon Quantification (>=2.1.0 - 2D - WSI (8-bit) only!)

CAM Assay (>=3.1.0 - WSI (8-bit) only!)

Cell Painting (>=1.0.0 - Multichannel - WSI (8/16-bit) only!)

Confluence Assay (>=1.0.0 - WSI (8-bit) only!; >=2.0.0)

Cytosolic & Nuclear Translocation Assay (>=3.1.0 - 2D/TIME SERIES - WSI only!)

Ki-67 Breast Quantification (>=3.1.0 - 2D - WSI (8-bit) only!)

Network Formation Assay (>=2.1.0 - WSI (8-bit) only!)

Neuronal Network Morphology (>=1.0.0 - WSI (8-bit) only!) (not a publicly available app)

Sparkfinder (>=1.0.0 - Multichannel - WSI - (8/16-bit) only!)

Spheroid Lipid Droplet Profiling (>=1.0.0 - Multichannel - WSI (8/16-bit) only!) (not a publicly available app)

Spheroid Sprouting Assay (>=2.1.0 - WSI (8-bit) only!)

Wound Healing (Scratch) Assay (>=2.1.0 - WSI (8-bit) only!, >=3.0.0)

Ovarian Carcinoma DAB Scoring (>=1.0.0 2D - TIME SERIES - WSI (8-bit) only!) (not a publicly available app)

CD31 IHC Vessel Quantification (>=1.0.0)

Neurite Outgrowth PC (>=1.0.0)

Brain FLARE M (>=1.0.0) (not a publicly available app)

IKOSA AI (deployed apps):

IKOSA AI Semantic Segmentation (>=4.2.0 - WSI (8-bit) only!, >=4.5.0)

IKOSA AI Instance Segmentation 2D (>=1.0.0 - WSI (8/16-bit) - only!)

IKOSA AI Instance Segmentation Multichannel (>=1.0.0 - Multichannel - WSI (8/16-bit) only!)

IKOSA AI Semantic Segmentation Multichannel (>=1.0.0 - Multichannel - WSI (8/16-bit) only!)

IKOSA AI (algorithm training):

IKOSA AI Semantic Segmentation (2D - WSI (8-bit) only!)

IKOSA AI Instance Segmentation 2D (2D - WSI (8/16-bit) only!)

IKOSA AI Instance Segmentation Multichannel (Multichannel - WSI (8/16-biT) only!)

IKOSA AI Semantic Segmentation Multichannel (Multichannel - WSI (8/16-bit) only!)

PreciPoint VMIC

vmic

image/x.precipoint.vmic

2D - WSI - 8-bit

Applications:

Axon Quantification (>=1.0.0)

CAM Assay (>=2.0.0)

Confluence Assay (>=1.0.0)

Cytosolic & Nuclear Translocation Assay (1.0.0, 2.0.0, 3.0.0, 3.1.0)

Ki-67 Breast Quantification (>=1.0.0)

Network Formation Assay (>=2.0.0)

Neuronal Network Morphology (>=1.0.0) (not a publicly available app)

Spheroid Sprouting Assay (2.0.0, 2.1.0)

Wound Healing (Scratch) Assay (>=1.0.0)

Ovarian Carcinoma DAB Scoring (>=1.0.0) (not a publicly available app)

CD31 IHC Vessel Quantification (>=1.0.0)

Neurite Outgrowth PC (>=1.0.0)

IKOSA AI (deployed apps):

IKOSA AI Semantic Segmentation (>=4.2.0)

IKOSA AI Instance Segmentation 2D (>=1.0.0)

IKOSA AI (application training):

IKOSA AI Semantic Segmentation

IKOSA AI Instance Segmentation 2D

PreciPoint GTIF

gtif

image/x.precipoint.gtif

2D - WSI - 8-bit

Applications:

Axon Quantification (>=1.0.0)

CAM Assay (>=2.0.0)

Confluence Assay (>=1.0.0)

Cytosolic & Nuclear Translocation Assay (1.0.0, 2.0.0, 3.0.0, 3.1.0)

Ki-67 Breast Quantification (>=1.0.0)

Network Formation Assay (>=2.0.0)

Neuronal Network Morphology (>=1.0.0) (not a publicly available app)

Spheroid Sprouting Assay (2.0.0, 2.1.0)

Wound Healing (Scratch) Assay (>=1.0.0)

Ovarian Carcinoma DAB Scoring (>=1.0.0) (not a publicly available app)

CD31 IHC Vessel Quantification (>=1.0.0)

Neurite Outgrowth PC (>=1.0.0)

IKOSA AI (deployed apps):

IKOSA AI Semantic Segmentation (>=4.2.0)

IKOSA AI Instance Segmentation 2D (>=1.0.0)

IKOSA AI (application training):

IKOSA AI Semantic Segmentation

IKOSA AI Instance Segmentation 2D

Generic Tiled TIFF (including an image pyramid)

tif, tiff

image/x.tiled.tiff+pyramid

2D - WSI - 8-bit

Applications:

Axon Quantification (>=1.0.0)

CAM Assay (>=2.0.0)

Confluence Assay (>=1.0.0)

Cytosolic & Nuclear Translocation Assay (1.0.0, 2.0.0, 3.0.0, 3.1.0)

Ki-67 Breast Quantification (>=1.0.0)

Network Formation Assay (>=2.0.0)

Neuronal Network Morphology (>=1.0.0) (not a publicly available app)

Spheroid Sprouting Assay (2.0.0, 2.1.0)

Wound Healing (Scratch) Assay (>=1.0.0)

Ovarian Carcinoma DAB Scoring (>=1.0.0) (not a publicly available app)

CD31 IHC Vessel Quantification (>=1.0.0)

Neurite Outgrowth PC (>=1.0.0)

IKOSA AI (deployed apps):

IKOSA AI Semantic Segmentation (>=4.2.0)

IKOSA AI Instance Segmentation 2D (>=1.0.0)

IKOSA AI (application training):

IKOSA AI Semantic Segmentation

IKOSA AI Instance Segmentation 2D

Generic Tiled TIFF (without an image pyramid)

tif, tiff

image/x.tiled.tiff

2D - WSI - 8-bit

Applications:

Axon Quantification (>=1.0.0 2D (8-bit)only)

CAM Assay (>=2.0.0 2D (8-bit)only)

Confluence Assay (>=1.0.0 2D (8-bit)only)

Cytosolic & Nuclear Translocation Assay (1.0.0, 2.0.0, 3.0.0, 3.1.0 2D (8-bit)only)

Ki-67 Breast Quantification (>=1.0.0 2D (8-bit)only)

Network Formation Assay (>=2.0.0 2D (8-bit)only)

Neuronal Network Morphology (>=1.0.0 2D (8-bit)only) (not a publicly available app)

Spheroid Sprouting Assay (2.0.0, 2.1.0 2D (8-bit)only)

Wound Healing (Scratch) Assay (>=1.0.0 2D (8-bit)only)

Ovarian Carcinoma DAB Scoring (>=1.0.0 2D (8-bit)only) (not a publicly available app)

CD31 IHC Vessel Quantification (>=1.0.0 2D (8-bit)only)

Neurite Outgrowth PC (>=1.0.0 2D (8-bit)only)

IKOSA AI (deployed apps):

IKOSA AI Semantic Segmentation (>=4.2.0 NO-WSI only!, >=5.3.0)

IKOSA AI Instance Segmentation 2D (>=1.0.0 NO-WSI only!, >=2.2.0)

IKOSA AI (application training):

IKOSA AI Semantic Segmentation

IKOSA AI Instance Segmentation 2D

Huron Digital Pathology TIFF

tif, tiff

image/x.huron.tiff+pyramid

2D - WSI - 8-bit

Important: except JPEG2000 compression

Applications:

Axon Quantification (>=1.0.0)

CAM Assay (>=2.0.0)

Confluence Assay (>=1.0.0)

Cytosolic & Nuclear Translocation Assay (1.0.0, 2.0.0, 3.0.0, 3.1.0)

Ki-67 Breast Quantification (>=1.0.0)

Network Formation Assay (>=2.0.0)

Neuronal Network Morphology (>=1.0.0) (not a publicly available app)

Spheroid Sprouting Assay (2.0.0, 2.1.0)

Wound Healing (Scratch) Assay (>=1.0.0)

Ovarian Carcinoma DAB Scoring (>=1.0.0) (not a publicly available app)

CD31 IHC Vessel Quantification (>=1.0.0)

Neurite Outgrowth PC (>=1.0.0)

IKOSA AI (deployed apps):

IKOSA AI Semantic Segmentation (>=4.2.0)

IKOSA AI Instance Segmentation 2D (>=1.0.0)

IKOSA AI (application training):

IKOSA AI Semantic Segmentation

IKOSA AI Instance Segmentation 2D

Philips Healthcare TIFF

tif, tiff

image/x.philips.tiff+pyramid

2D - WSI - 8-bit

Applications:

Axon Quantification (>=1.0.0)

CAM Assay (>=2.0.0)

Confluence Assay (>=1.0.0)

Cytosolic & Nuclear Translocation Assay (1.0.0, 2.0.0, 3.0.0, 3.1.0)

Ki-67 Breast Quantification (>=1.0.0)

Network Formation Assay (>=2.0.0)

Neuronal Network Morphology (>=1.0.0) (not a publicly available app)

Spheroid Sprouting Assay (2.0.0, 2.1.0)

Wound Healing (Scratch) Assay (>=1.0.0)

Ovarian Carcinoma DAB Scoring (>=1.0.0) (not a publicly available app)

CD31 IHC Vessel Quantification (>=1.0.0)

Neurite Outgrowth PC (>=1.0.0)

IKOSA AI (deployed apps):

IKOSA AI Semantic Segmentation (>=4.2.0)

IKOSA AI Instance Segmentation 2D (>=1.0.0)

IKOSA AI (application training):

IKOSA AI Semantic Segmentation

IKOSA AI Instance Segmentation 2D

Roche / Ventana TIFF

tif, tiff

Important: bif, vtif are NOT supported

image/x.ventana.tiff+pyramid

2D - WSI - 8-bit

Applications:

Axon Quantification (>=1.0.0)

CAM Assay (>=2.0.0)

Confluence Assay (>=1.0.0)

Cytosolic & Nuclear Translocation Assay (1.0.0, 2.0.0, 3.0.0, 3.1.0)

Ki-67 Breast Quantification (>=1.0.0)

Network Formation Assay (>=2.0.0)

Neuronal Network Morphology (>=1.0.0) (not a publicly available app)

Spheroid Sprouting Assay (2.0.0, 2.1.0)

Wound Healing (Scratch) Assay (>=1.0.0)

Ovarian Carcinoma DAB Scoring (>=1.0.0) (not a publicly available app)

CD31 IHC Vessel Quantification (>=1.0.0)

Neurite Outgrowth PC (>=1.0.0)

IKOSA AI (deployed apps):

IKOSA AI Semantic Segmentation (>=4.2.0)

IKOSA AI Instance Segmentation 2D (>=1.0.0)

IKOSA AI (application training):

IKOSA AI Semantic Segmentation

IKOSA AI Instance Segmentation 2D

Leica SCN

(Brightfield)

scn

image/x.leica.scn.tiff+pyramid

2D - WSI - 8-bit

Applications:

Axon Quantification (>=1.0.0)

CAM Assay (>=2.0.0)

Confluence Assay (>=1.0.0)

Cytosolic & Nuclear Translocation Assay (1.0.0, 2.0.0, 3.0.0, 3.1.0)

Ki-67 Breast Quantification (>=1.0.0)

Network Formation Assay (>=2.0.0)

Neuronal Network Morphology (>=1.0.0) (not a publicly available app)

Spheroid Sprouting Assay (2.0.0, 2.1.0)

Wound Healing (Scratch) Assay (>=1.0.0)

Ovarian Carcinoma DAB Scoring (>=1.0.0) (not a publicly available app)

CD31 IHC Vessel Quantification (>=1.0.0)

Neurite Outgrowth PC (>=1.0.0)

IKOSA AI (deployed apps):

IKOSA AI Semantic Segmentation (>=4.2.0)

IKOSA AI Instance Segmentation 2D (>=1.0.0)

IKOSA AI (application training):

IKOSA AI Semantic Segmentation

IKOSA AI Instance Segmentation 2D

PerkinElmer QPTIFF

qptiff

image/x.perkinelmer.qptiff+pyramid

2D - WSI - 8-bit

Multichannel - WSI - 8-bit

Multichannel - WSI - 16-bit

Applications:

Axon Quantification (>=1.0.0 - 2D - WSI (8-bit) only!)

CAM Assay (>=2.0.0 - 2D - WSI (8-bit) only!; >=3.2.0 - WSI (8-bit) only!)

Cell Painting (>=1.0.0 - Multichannel - WSI (8/16-bit) only!)

Confluence Assay (1.0.0 - 2D - WSI (8-bit) only! >=1.1.0 - WSI (8-bit) only!; >=2.0.0)

Cytosolic & Nuclear Translocation Assay (1.0.0, 2.0.0, 3.0.0, 3.1.0 - 2D - WSI (8-bit) only!)

Ki-67 Breast Quantification (>=1.0.0 - 2D - WSI (8-bit) only!)

Network Formation Assay (>=2.0.0 - 2D - WSI (8-bit) only!; >=2.2.0 - WSI (8-bit) only!)

Neuronal Network Morphology (>=1.0.0 - WSI (8-bit) only!) (not a publicly available app)

Sparkfinder (>=1.2.0 - Multichannel - WSI (8/16-bit) only!)

Spheroid Lipid Droplet Profiling (>=1.0.0 - Multichannel - WSI (8/16-bit) only!) (not a publicly available app)

Spheroid Sprouting Assay (2.0.0, 2.1.0 - 2D - WSI (8-bit) only!; >=2.2.0 - WSI (8-bit) only!)

Wound Healing (Scratch) Assay (>=1.0.0 - 2D - WSI (8-bit) only!; >=2.2.0 - WSI (8-bit) only!; >=3.0.0)

Ovarian Carcinoma DAB Scoring (>=1.0.0 2D/TIME SERIES - WSI (8-bit) only!) (not a publicly available app)

CD31 IHC Vessel Quantification (>=1.0.0 2D/TIME SERIES - WSI (8-bit) only!)

Neurite Outgrowth PC (>=1.0.0)

Brain FLARE M (>=1.0.0) (not a publicly available app)

IKOSA AI (deployed apps):

IKOSA AI Semantic Segmentation (>=4.2.0 - 2D - WSI (8-bit) only!; >=5.1.0)

IKOSA AI Instance Segmentation 2D (>=1.0.0 - 2D - WSI (8-bit) only!; >=2.1.0)

IKOSA AI Instance Segmentation Multichannel (>=2.1.0 -Multichannel - WSI (8/16-bit) only!)

IKOSA AI Semantic Segmentation Multichannel (>=1.0.0 -Multichannel - WSI (8/16-bit) only!)

IKOSA AI (application training):

IKOSA AI Semantic Segmentation (2D - WSI (8-bit) only!)

IKOSA AI Instance Segmentation 2D (2D - WSI (8-bit) only!)

KOSA AI Instance Segmentation Multichannel (Multichannel - WSI (8/16-bit) only!)

IKOSA AI Semantic Segmentation Multichannel (Multichannel - WSI (8/16-bit) only!)

JPEG file (JFIF encoded)

jpg, jpeg

image/jpeg

2D - 8-bit

Applications:

Axon Quantification (>=1.0.1)

CAM Assay (>=1.0.0)

CAM Grid Assay (1.0.0, 1.1.0)

Confluence Assay (>=1.0.0)

Cytosolic & Nuclear Translocation Assay (1.0.0, 2.0.0, 3.0.0, 3.1.0)

Fibrin Tube Formation Assay (1.0.0, 1.0.2)

Fungi Repaint Assay (1.0.0, 2.0.0)

Ki-67 Breast Quantification (>=1.0.0)

Network Formation Assay (>=1.0.0)

Neuronal Network Morphology (>=1.0.0) (not a publicly available app)

Spheroid Sprouting Assay (1.0.0, 2.0.0, 2.1.0)

TEM Myocardium Assay (1.0.0)

Wound Healing (Scratch) Assay (>=1.0.0)

Ovarian Carcinoma DAB Scoring (>=1.0.0) (not a publicly available app)

CD31 IHC Vessel Quantification (>=1.0.0)

Neurite Outgrowth PC (>=1.0.0)

IKOSA AI (deployed apps):

IKOSA AI Semantic Segmentation (>=4.2.0)

IKOSA AI Instance Segmentation 2D (>=1.0.0)

IKOSA AI (application training):

IKOSA AI Semantic Segmentation

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