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If it is not on the list of available applications, please contact your organization's administrator or our team at ","type":"text"},{"text":"support@ikosa.ai","type":"text","marks":[{"type":"link","attrs":{"href":"mailto:support@ikosa.ai"}}]},{"text":".","type":"text"}]},{"type":"heading","attrs":{"level":1},"content":[{"text":"Application description","type":"text"}]},{"type":"paragraph","content":[{"text":"This application automatically measures the (artificial) gap, the so-called “scratch”, in images showing a cell monolayer. In addition to the scratch width, areas that are (not) covered with cells are quantified (including/excluding single cells or cell-free areas). The application supports images from phase contrast and brightfield microscopy and arbitrary cell types. This analysis can also be performed on time lapse recordings (time series) uploaded as 8-bit multipage TIFF files to study directional cell migration in vitro, z-stacks, or multichannel images, uploaded as 8-bit multipage TIFF files.","type":"text"}]},{"type":"paragraph","content":[{"text":"In the following sections, we provide the necessary input data requirements that are necessary to obtain accurate image analysis results and a description of the output files.","type":"text"}]},{"type":"heading","attrs":{"level":1},"content":[{"text":"Input data requirements","type":"text"}]},{"type":"heading","attrs":{"level":2},"content":[{"text":"Input image(s)","type":"text"}]},{"type":"paragraph","content":[{"text":"Input for this application is the following image 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