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  • Create a label set including at least 1 label (e.g. for a specific cell or tissue type) and

  • Annotate at least 2 images, meaning ALL all relevant objects you would like to segment have to be annotated and labelled (e.g. all lipid droplets, mitochondria as shown in the figures below)

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Note

Important: Make sure these images contain ONLY only background, as unlabeled features introduce counterfactual information into the trained algorithm. Make sure that all objects in the selected images/ROI(s) are annotated and labelled correctly.

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